By Jackie Bellamy-Zions
Guelph, ON Aug. 8, 2018 - While most of us are more concerned with eliminating waste, the examination of manure and gut contents are providing fascinating upcoming studies for two Ontario Veterinary College researchers, Drs Scott Weese and Luis Arroyo. The research is made possible by the Ontario Equestrian Member Equine Research Fund, which was recently established by Ontario Equestrian (OE), who asked their members where they thought the most benefit would be gained.
“The microbial communities of the gut play a crucial role in the health of the horse, and we now know there are major differences between the gut microbiota of healthy horses and those with colitis,” says Arroyo. Both Arroyo and Weese understand the importance of researching what is “normal” when it comes to the horse’s microbiota before links can be made between microbiota changes and disease. In the most recent publication of the Equine Acute Abdomen, Weese writes: The gut microbiota plays critical roles in nutrition, metabolism, and a wide range of other functions and is absolutely required for health; however, it can also be involved with, or a direct cause of, a myriad of diseases.
“At Ontario Equestrian, we recognize the importance of a healthy horse to our sport - not to mention the importance of educating our riders and members, and Equine Guelph’s international reputation for excellence in Equine Health, Research and Education made them an obvious partner for us to support ongoing research for our members and their equine partners,” says Ontario Equestrian Executive Director Tracey McCague-McElrea. “We’re excited to see this partnership develop in its inaugural year, and we look forward to sharing the results of Dr. Weese and Dr. Arroyo’s research with our members.”
Simulated Gut Study: Rogogut
“The funding from Ontario Equestrian is very timely and crucial for the acquisition of specialized equipment, material and supplies needed to achieve our goals,” says Arroyo. “I am deeply grateful for their support of this research program and the investment to this devastating equine illness, colitis.” The microbial communities of the gut play a crucial role in the health of the horse, and we know now that there are major differences between the gut microbiota of healthy horses and those with colitis. In more recent years, mimicking the growth environment and nutritional conditions of the natural habitat of bacteria has revolutionized traditional bacterial culture. The bacteria populations (particularly the anaerobes) within the large colon can now be better characterized in health and disease by combining culture enriched-based methods and molecular profiling of intestinal contents of horses with or without colitis. With the help of Dr. Emma Allen-Vercoe (designer of the human Robogut), Arroyo is setting up a simulated gut or Robogut to help understand what a healthy horse microbiome looks like. Just as scientists now believe that many cases of colitis in humans are due to imbalances in the microbiome, and not pathogens as was previously thought; these findings are guiding the research into the microbiome and colitis in horses.
On Poop Patrol
Weese and his team are looking to find out how much the “normal” horse’s microbiota changes, over the course of a year, with a study that will frequently collect and examine fecal samples from 15 to 20 horses.
“It is not always easy to find money for studies establishing fundamental baselines,” says Weese, “This funding from Ontario Equestrian is very important so we can have confidence in our interpretations for future disease studies.”
By analyzing samples from healthy horses over the course of a year they will learn if the microbiota are impacted by seasonal changes, gain insight on different diets and how they affect the microbial population, and study composition versus function of microbiota. Weese proposes from horse to horse, it may be possible to have completely different bugs performing the exact same functions.
“When it comes to diagnosing disease, at the moment there is not enough knowledge of the equine intestinal microbiota to determine the difference between incidental or cause and effect links,” says Weese.
In humans there are links between endocrine disease, obesity, and gut function. There is also great interest in establishing links between the gut microbiota and metabolic diseases in horses, with finding just starting to emerge. We typically think of the gut in terms of colic, laminitis, and colitis but there are likely many more things equine gut microbiota can influence or be impacted by such as insulin resistance or gastrointestinal disease following antibiotic administration. The future is exciting with the possibilities of restoration of normal microbiota as a reasonable clinical goal for prevention or treatment. But “first things first” for Weese means getting the scoop on poop for the baselines of “normal” microbiota.
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